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Paraprobiotics, postbiotics and other -biotics
Crha, Richard ; Šálek, Petr (referee) ; Trachtová, Štěpánka (advisor)
The theoretical part of the thesis deals with a wide range of microorganisms and substances derived from probiotics. Based on recent publications, probiotics, prebiotics, synbiotics, paraprobiotics, postbiotics, pharmabiotics, psychobiotics and oncobiotics are defined. Furthermore, the purpose and use of the mentioned -biotics is specified for the current understanding of their function. In the experimental part, DNA was isolated from a sample of probiotic capsules using a commercially available bacterial kit and magnetic particles. Its purity and concentration were then determined spectrophotometrically. Using the isolated DNA as a matrix for conventional PCR and detection on agarose gel, the composition of the probiotic product as declared by the manufacturer was demonstrated. Finally, the PMA-PCR method was optimized.
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The role of intestinal microbiota in the development of Alzheimer's dementia
Hakenová, Kristina ; Valeš, Karel (advisor) ; Vodička, Martin (referee)
Preserving health at a higher age depends on the function of biological homeostatic systems (neural endocrine and immune activities), where the biological interaction between these systems and the intestinal microflora plays a key role. One of the most serious diseases associated with aging is Alzheimer's dementia (AD) and it is the most common cause of dementia in the world. This disease is accompanied by cognitive impairment associated with accumulation of amyloid plaques and neurofibrillary fibres in brain tissue. Naturally, biological changes occur during the process of aging, resulting in reduced functional capacity of the organism, accompanied by an increased risk of inflammatoryprocesses involved in the etiology of several chronic, pathologies, including neurodegenerative processes. These modulatory processes are associated with oxidative stress and intestinal dysbiosis. It is already well documented in the scientific literature that significant changes in the composition of intestinal microbiota occur in aging. These changes can lead to several biological changes, including increased permeability of the intestinal barrier, thereby causing the increased penetration of many biologically active substances into the body. This may be a risk factor for the development of inflammation in both...
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The role of intestinal microbiota in the development of Alzheimer's dementia
Hakenová, Kristina ; Valeš, Karel (advisor) ; Vodička, Martin (referee)
Preserving health at a higher age depends on the function of biological homeostatic systems (neural endocrine and immune activities), where the biological interaction between these systems and the intestinal microflora plays a key role. One of the most serious diseases associated with aging is Alzheimer's dementia (AD) and it is the most common cause of dementia in the world. This disease is accompanied by cognitive impairment associated with accumulation of amyloid plaques and neurofibrillary fibres in brain tissue. Naturally, biological changes occur during the process of aging, resulting in reduced functional capacity of the organism, accompanied by an increased risk of inflammatoryprocesses involved in the etiology of several chronic, pathologies, including neurodegenerative processes. These modulatory processes are associated with oxidative stress and intestinal dysbiosis. It is already well documented in the scientific literature that significant changes in the composition of intestinal microbiota occur in aging. These changes can lead to several biological changes, including increased permeability of the intestinal barrier, thereby causing the increased penetration of many biologically active substances into the body. This may be a risk factor for the development of inflammation in both...
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